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Standard Operating Procedures (SOPs)

LCMS recognize that safety is a very important element when working in a laboratory environment. Please read all of the following sections carefully and do not hesitate to contact Andrea Fleig if you have any questions.

  1. General Lab Procedures
    1. Mandatory Research Training and Certification
    2. Safe Laboratory Practices
    3. Laboratory Waste Disposal
    4. Sterile Technique and Hood Work
    5. Pipettes Technique and Handling
    6. Solution Preparation
    7. Labeling of Solutions/Chemicals
    8. Chemical Inventory
    9. Autoclaving
    10. Laundering Lab Coats
    11. Spill Clean-Up Procedures
      1. Biohazardous Liquids
      2. Chemicals
    12. Check-Out Checklist
    13. Select Agent Toxin, Safe Handling, Storage and Disposal
    14. Biological Commodity Transport SOP
      1. BSP2 permit SOP
      2. Download the partially filled BSP2 form
    15. Reporting Laboratory Injuries
  2. Ordering Procedures
    1. LCMS Online Ordering
    2. Receiving Packages
    3. Removal of old/broken equipment
    4. Purchasing (ask Mahea or Andrea for document)
    5. Equipment Purchases above $1500 (ask Mahea or Andrea for document)
  3. Lab and Equipment Maintenance
    1. Lab Equipment Maintenance Checklist
    2. Replacing the CO2 Gas Cylinders
    3. Using the Miele dishwashers
    4. Temperature Monitoring System
    5. Osmometers
    6. pH meters
    7. Incubators
  4. Tissue Culture
    1. Maintenance of Cell Cultures
      1. Transient Transfection (ask Mahea or Andrea for document)
      2. Establishment of Stable Cell Lines (ask Mahea or Andrea for document)
      3. Cell Counting (ask Mahea or Andrea for document)
      4. Differential Staining of Cells (ask Mahea or Andrea for document)
      5. Cell Culture Biochemistry (ask Mahea or Andrea for document)
    2. Freezing and Thawing Cells
      1. Cryopreservation of Cell Lines
      2. Resuscitation of Frozen Cell Lines
    3. Subculture of Cell Lines
    4. Cell Lines
  5. Animal Procedures
    1. General Animal Use Policy
    2. Mouse Colony Management
    3. Mouse Breeding and Weaning
    4. Rodent Ear Notching and Tail Snipping
    5. Animal Transfers
    6. Genotyping TRPM2 Mice
    7. Genotyping STIM2 Mice
    8. Genotyping AKA Mice
    9. Animal Disposal
    10. Animal Handling In Disaster Response Plan
  6. Cell and Molecular Biology
    1. PCR
    2. Mutagenesis
    3. Agarose Gel Electrophoresis
    4. Gel Extraction
    5. DNA Purification
    6. Restriction Digest
    7. DNA Ligation
    8. Transformation
    9. DNA Sequencing
    10. Transfection (e.g., ORAI1 or TRPM6)
      1. TRPM6 cDNA transfection (ZZ)
      2. PEI Transfection (HY)
    11. RNA Purification
    12. cDNA Synthesis for RT-PCR
    13. Western Blotting
      1. Co-Immunoprecipitation
    14. Quantitation of Nucleic Acids
    15. Solutions
    16. cDNA Plasmid Inventory
    17. Antibody Inventory
    18. BrdU Assay
    19. ELISA (ask Mahea or Andrea for document)
    20. Flex Station Assay (ask Mahea or Andrea for document)
    21. ViCell (ask Mahea or Andrea for document)
    22. MTT assay (ask Mahea or Andrea for document)
    23. Annexin/VPI assay (ask Mahea or Andrea for document)